2nd November 2019

microbehunter
15

Why do you need to hold a slide at a 45 angle?

Why is it important to apply a cover slip at a 45 degree angle when making a wet mount? Applying the cover slip at an angle (instead of dropping it down flat on the specimen) pushes the air to the side and therefore minimizes the risk of air bubbles.

Also asked, how do you use the oil immersion lens?

Place a small drop of immersion oil 10 on the center of the viewing area of the slide. Continue to rotate the nosepiece so that the 100X objective is rotated into the oil. Use only the fine focus and refocus your specimen. Determine the shapes of the bacteria and draw them in the place provided.
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