Why do we use HCL in mitosis?

4 – the purpose of the hydrochloric acid is to destroy the substances that unite the cells (usually pectin), but it does not destroy the cell walls. The hydrochloric acid also has the ability to kill the cells and halt the process of mitosis. Photographs of garlic cells in different stages of mitosis follow.
A.

What is Orcein stain used for?

Orcein is a reddish-brown dye, orchil is a purple-blue dye. Orcein is also used as a stain in microscopy to visualize chromosomes, elastic fibers, Hepatitis B surface antigens, and copper-associated proteins.
  • What Colour does acetic Orcein stain?

    Aceto-orcein stain turns chromosomes a purple-red colour. This stain can be prepared from powder or purchased as solution; staining is best with freshly prepared stain as, over time, the stain precipitates and changes colour from a deep red or burgundy colour to a brownish colour.
  • What has the highest mitotic index?

    From the following list, which normal tissues would you expect to have the highest mitotic index: muscle, skin, kidney, or lung? Explain your answer. I believe skin has the highest mitotic index because you have to constantly replace skin cells.
  • Which type of cancer is the fastest growing and why?

    Which type of cancer is the fastest growing? Explain your answer, using your relevant data. -Ovarian cancer is the fastest growing due to the high mitotic index.
B.

What is Acetocarmine stain used for?

Acetocarmine staining. To stain plant chromosomes, a 1% solution of carmine in 45% acetic acid is used. Freshly fixed material is transferred into 1% acetocarmine for at least 30 min and then analyzed by the squash method.
  • Are proteins Acidophilic or Basophilic?

    Basophilic and acidophilic staining. Acidic dyes react with cationic or basic components in cells. Proteins and other components in the cytoplasm are basic, and will bind to acidic dyes. Another way of saying this is that cytoplasmic proteins are acidophilic (acid liking - i.e. bind to acidic dyes).
  • What is meant by Schiff's base?

    : any of a class of bases of the general formula RR′C=NR″ that are obtained typically by condensation of an aldehyde or ketone with a primary amine (as aniline) with elimination of water, that usually polymerize readily if made from aliphatic aldehydes, and that are used chiefly as intermediates in organic synthesis
  • What is an Aldimine?

    In organic chemistry, an aldimine is an imine that is an analog of an aldehyde. As such, aldimines have the general formula R–CH=N–R'. Aldimines are similar to ketimines, which are analogs of ketones.
C.

Why do we use Acetocarmine in mitosis?

The most commonly used root tips in labs to study mitosis are onion, wheat, lentil, barley and alfalfa. The super coiled chromosomes during different stages of mitosis present in the onion root tip cells can be visualized by treating with DNA specific stains, like Feulgen stain and Acetocarmine stain.
  • Why do we go through mitosis?

    The main functions of mitosis are growth and repair. Some cells once fully formed do not undergo cell division, such as nerve cells and muscle cells. Since you can never re-grow or repair these types of cells once they are mature, you must take care of the ones you have.
  • Is an onion a root?

    Potato – This is a stem, an underground storage stem called a tuber. Carrot – This is a modified tap root, for storage. Onion layers – The bulb of the onion contains both a modified stem, in the center, but most of what we eat are layers that are modified underground leaves.
  • What does mitosis lead to?

    1. Explain how mitosis leads to two daughter cells, each of which is diploid and genetically identical to the original cell. Mitosis leads to two daughter cells when the DNA is duplicated and the cell splits. THe cells goes through interphase, prophase, metaphase, anaphase, and telophase.

Updated: 20th September 2018

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