Why do we sometimes stain cells before looking at them through the microscope?
The most basic reason that cells are stained is to enhance visualization of the cell or certain cellular components under a microscope. Cells may also be stained to highlight metabolic processes or to differentiate between live and dead cells in a sample.
Staining is an auxiliary technique used in microscopy to enhance contrast in the microscopic image. Stains and dyes are frequently used in biology and medicine to highlight structures in biological tissues for viewing, often with the aid of different microscopes.
- Cytoplasm, the rest of the material of the cell within the plasma membrane, excluding the nucleoid region or nucleus, that consists of a fluid portion called the cytosol and the organelles and other particulates suspended in it. Ribosomes, the organelles on which protein synthesis takes place.
- A counterstain, safranin, is then used to give color to the nonsporeforming bacteria. At the end of the procedure, spores stain green and other cells stain red. The Gram stain procedure used for differentiating bacteria into two groups. Another differential stain technique is the acid-fast technique.
- (4 points) Specimens viewed with a compound microscope must be very thin, so that light can pass through them. If they were not thin enough for light to pass through them, they would not be visible under a compound microscope.
Updated: 3rd December 2019