Homologous chromosomes can have different alleles on them. Alleles are variants of the same gene that occur on the same place on a chromosome. (Through a mutation, they are different.) A locus refers to the location on the chromosome where the gene is found.
Similarly one may ask, do alleles make up genes?
In a diploid organism, one that has two copies of each chromosome, two alleles make up the individual's genotype. An example is the gene for blossom color in many species of flower — a single gene controls the color of the petals, but there may be several different versions (or alleles) of the gene.
How are genes and alleles related to each other?
An allele is a form of a gene. For example, in humans, the ABO gene for the ABO blood group is located on chromosome 9, (http://en.wikipedia.org/wiki/ABO_blood_group_system) and has three alleles (forms), A, B, and O. A person can inherit only two alleles for the ABO blood group, one from each parent.