Hershey and Chase used T2 phage, a bacteriophage. The phage infects a bacterium by attaching to it and injecting its genetic material into it. They put labels on phage DNA with radioactive Phosphorus-32. They then followed the phages while they infected E. coli.
Similarly, you may ask, why was sulfur and phosphorus used in Hershey's experiment?
Why were phosphorus-32 and sulfur-35 perfect for what Hershey & Chase were investigating in their experiments? They were perfect because proteins contain almost no phosphorus and DNA contains no sulfur, so the radioactive isotopes would show up in the cells if the phosphorus-32 or sulfur-35 appeared.
Why were Hershey and Chase's experiments so important?
Hershey-Chase experiment: An extraordinarily important experiment in 1952 that helped to convince the world that DNA was the genetic material. After a phage particle attaches to a bacterium, its DNA enters through a tiny hole while its protein coat remains outside.
Why was a blender used in the Hershey and Chase experiment?
After introducing to the phage culture to the bacterial sample, they used a Waring blender to violently disturb the infected bacteria, causing the protein shells to detach from their hosts. Then, using a centrifuge, they separated the bacterium from the phages and protein. The Hershey-Chase Blender Experiment.