What is the purpose of the ethanol in a DNA extraction?


Updated: 2nd October 2019

Ethanol precipitation is a commonly used technique for concentrating and de-salting nucleic acids (DNA or RNA) preparations in aqueous solution. The basic procedure is that salt and ethanol are added to the aqueous solution, which forces the precipitation of nucleic acid nucleic acids out of solution.

Why do we use protease in DNA extraction?

For one, proteases catalyze the breakdown of contaminating proteins present in the solution to its component amino acids. It also degrades any nucleases and/or enzymes that may be present in the sample. This is of vital importance since these chemical compounds can attack and destroy the nucleic acids in your sample.

What is the difference between protease and proteinase?

A protease (also called a peptidase or proteinase) is an enzyme that performs proteolysis: protein catabolism by hydrolysis of peptide bonds.
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