Typically, a DNA molecule is digested with restriction enzymes, and the agarose gel electrophoresis is used as a diagnostic tool to visualize the fragments. An electric current is used to move the DNA molecules across an agarose gel, which is a polysaccharide matrix that functions as a sort of sieve.
Similarly, who uses gel electrophoresis?
Gel electrophoresis is a technique used to separate DNA fragments (or other macromolecules, such as RNA and proteins) based on their size and charge. Electrophoresis involves running a current through a gel containing the molecules of interest.
What is gel electrophoresis and what is it used for?
Gel electrophoresis is a technique commonly used in laboratories to separate charged molecules like DNA?, RNA? and proteins? according to their size. The movement of charged molecules is called migration. Molecules migrate towards the opposite charge.
Why do forensic scientists use gel electrophoresis?
The Use of Electrophoresis in Forensic Science. In electrophoresis, chemicals such as blood proteins, DNA or inorganic ions can be separated according to differences in their mass and/or charge. The solid medium used in electrophoresis is usually an agarose or polyacrylamide gel.