16th October 2019

wikipedia

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What is the pipette used for?

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A pipette (sometimes spelled pipet) is a laboratory tool commonly used in chemistry, biology and medicine to transport a measured volume of liquid, often as a media dispenser. Measurement accuracy varies greatly depending on the style.

So, what is the calibration of a pipette?

The calibration of pipette is carried out by gravimetric method. When determining the volume of water, the accuracy of measurements is effected by ambient temperature, atmospheric pressure and relative humidity. These factors are usually combined to give the Z factor, used in calculation of volume of water.

Why is it important to calibrate your pipette?

Pipette calibration is important for good laboratory practice and is a vital part of any lab regime where precise volumes of fluid need transferring or diluting. Environmental factors such as warmer or colder temperatures and humidity play a role as well as the temperature and viscosity of the test fluids.

What is calibration glassware?

CHEM 334 Quantitative Analysis Laboratory. Calibration of Volumetric Glassware. Introduction. Volumetric glassware is a class of glass vessels that are calibrated to contain or deliver certain volumes of substances.

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How does the pipette work?

The liquid, in contrast, is taken up into a disposable pipette tip and then dispensed. The piston moves the air cushion and the liquid is thus taken up into the pipette tip or dispensed out of it. The air cushion thus works like an elas- tic spring, to which the liquid sticks.

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What is pipetting technique?

Use this technique when pipetting viscous liquids or volatile solvents. Reverse pipetting also helps when pipetting ultra-micro samples of 0.5 µL or less. Push the piston down to the purge position (the second stop), then draw the liquid up.

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What is a measuring pipette?

The "volumetric" or "transfer" pipette measure a particular volume to high accuracy. (The three pipettes at the top of Figure 1 are volumetric pipettes designed to deliver 25, 20 and 10 mL, respectively.) Volumetric pipettes are designed such after a fluid is dispensed, a small drop of liquid will remain in the tip.

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Are pipettes accurate?

A pipette is accurate to the degree that the volume delivered is equal to the specified volume. Precision, on the other hand, is concerned with the closeness of several measurements to each other, rather than to a standard value, that is the reproducibility of the pipetting samples.

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What is the use of graduated pipette?

Graduated pipette. A slender, calibrated tube used in laboratory to measure the volume or transfer a particular quantity of liquid from one container to another. Calibration marks are confined along the length of the stem (as in mohr pipette), or extend to the tip (as in serologic pipette).

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What is a pipette tip?

Pipette tips, sometimes spelled pipet tips, are used with pipettes and pipettors to speed processing and reduce cross-contamination. They are available in a variety of materials and styles. Universal pipette tips are uses with most standard-diameter pipettes. They are usually made of polypropylene and disposable.

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What is a pipette pump?

…dispenser pipette pumps provide safe and accurate pipetting and dispensing of liquids from serological pipettes. Glass or plastic pipettes are inserted into the tapered collar and the thumbwheel is rotated for precision filling or dispensing. The elastic chuck in the collar secures the pipette to…

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What is a micropipette used for?

Pipettes and micropipettes are used to measure and deliver accurate volumes of liquid. The difference between the two is that micropipettes measure a much smaller volume, starting at 1 microliter, while pipettes generally start at 1 milliliter.

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What is the use of a dropper?

Pasteur pipettes, also known as droppers or eye droppers, are used to transfer small quantities of liquids. They are usually glass tubes tapered to a narrow point, and fitted with a rubber bulb at the top. The combination of the Pasteur pipette and rubber bulb has also been referred to as a teat pipette.

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Who created the pipette?

The micropipette was invented in 1957 at University of Marburg, Germany by postdoc Heinrich Schnitger. Frustrated by repetitive pipetting of small volumes using glass micropipettes, Schnitger developed a prototype with a spring-loaded piston and a removable plastic tip for containing liquid.

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What are burettes used for?

Burette, also spelled Buret, laboratory apparatus used in quantitative chemical analysis to measure the volume of a liquid or a gas. It consists of a graduated glass tube with a stopcock (turning plug, or spigot) at one end.

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What is the use of the conical flask?

They come in a range of sizes, materials and uses. Commonly used laboratory flasks include: An Erlenmeyer flask, conical, or E-flask is one of the most commonly used flasks in scientific laboratories. The flask has a conical base that almost extends into a small, cylindrical neck.

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What is the use of a ring stand?

A retort stand, sometimes called a clamp stand or a ring stand, is a piece of scientific equipment, clamps can be attached to hold other pieces of equipment. For instance, burettes, commonly used for titration experiments, test tubes and flasks.

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What is the test tube used for?

A test tube is a clear glass or plastic container that is much longer than it is wide, commonly has a U-shaped bottom, and has an open top. Test tubes are used to hold, mix, and heat chemical experiments. They are used as homes for microorganisms when people want to culture (grow) them.

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What is the use of a Scoopula?

Scoopula is a brand name of a spatula-like scoop utensil used primarily in chemistry lab settings to transfer solids: to a weigh paper for weighing, to a cover slip to measure melting point, or a graduated cylinder, or to a watch glass from a flask or beaker through scraping.

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What is a Erlenmeyer flask used for in a laboratory?

The Erlenmeyer flask was created by Emil Erlenmeyer, a German chemist, in the 1860s. He developed a flask that has a flat bottom with a tapered neck. The mouth, or opening, is used for pouring solutions and may be closed with a stopper in order to store samples.

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What is a pipette in forex?

Many forex platforms use pips as the smallest fraction that currencies pairs can move but the need for more accuracy has produced fractions of one pip which are called pipettes. A pipette equals 1/10 (one tenth) of a pip and it represents a fraction of 1/100,000 (one in hudrend thousand).

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How do micropipettes work?

Regardless of the manufacturer, micropipettes operate on the same principle: a plunger is depressed by the thumb and as it is released, liquid is drawn into a disposable plastic tip.

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What is the definition of a graduated cylinder?

A graduated cylinder, measuring cylinder or mixing cylinder is a common piece of laboratory equipment used to measure the volume of a liquid. It has a narrow cylindrical shape. Each marked line on the graduated cylinder represents the amount of liquid that has been measured.

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