What is the optimal pH for trypsin activity?
Effects of pH
|Lipase (stomach)||4.0 - 5.0|
|Lipase (castor oil)||4.7|
|Pepsin||1.5 - 1.6|
|Trypsin||7.8 - 8.7|
Aim: To find out the effect of temperature on the activity (rate of reaction) catalysed by the enzyme trypsin. Hypothesis: The milk will turn translucent fastest at around 50°C. The enzyme trypsin generally reaches its optimum at around 50°C.
- As the temperature increases, so does the rate of reaction. But very high temperatures denature enzymes. The graph shows the typical change in an enzyme's activity with increasing temperature. The enzyme activity gradually increases with temperature up to around 37ºC, or body temperature.
- Trypsin (EC 22.214.171.124) is a serine protease from the PA clan superfamily, found in the digestive system of many vertebrates, where it hydrolyzes proteins. Trypsin is formed in the small intestine when its proenzyme form, the trypsinogen produced by the pancreas, is activated.
- CONCLUSION: The optimal temperature in alimentary tract of Wh. pigra for lipase and amylase was 37 degrees C, and the responding temperature for protease was 42 degrees C. The optimal pH value in alimentary tract of Wh. pigra for lipase and amylase was pH 8.2 and pH 5.2, respectively.
Pepsin works in the highly acidic conditions of the stomach. It has an optimum pH of about 1.5. On the other hand, trypsin works in the small intestine, parts of which have a pH of around 7.5. Trypsin's optimum pH is about 8.
- The pH-dependence of the peptidase activity of aminoacylase. Aminoacylase is a potent peptidase around pH 8.5. The pH dependence of the Km values reveals that only dipeptides with uncharged N-terminal amino acids are substrates of the enzyme.
- Optimum is a useful word which means "the best". We call the temperature or pH which makes an enzyme work at its very fastest the optimum for that enzyme. The rate of enzyme activity increases with as temperature increases until the optimum temperature, then falls to zero as the enzyme is denatured.
- The enzymes there work at different optimum pH levels. For example: salivary amylase works best just below pH 7. stomach protease works best at around pH 1.6.
Updated: 17th October 2019