What is in a tiger top tube?
Gold or red/gray tube, also known as a tiger tube that contains a clot activator. It is used to separate blood from the serum. Tubes with a red stopper are used to collect serum to test for routine donor screening or infectious disease. It contains a clot activator.
This tube is used for preparing EDTA plasma, whole blood, and bone marrow specimens. This tube is used for collecting serum or clotted whole blood specimens. Tiger-top tube (serum separator): Tube does not contain an anticoagulant but does contain a clot activator and serum separator gel.
- Blood outside the body with anticoagulant is referred to as whole blood. Whole blood can still be separated into liquid and cells. This liquid is called plasma. It is the liquid portion of blood when the tube contains anticoagulant.
- LIGHT BLUE. Citrate is an anticoagulant which binds calcium in the blood. Since it is bound up the blood cannot clot resulting in a whole blood sample, red blood cells and PLASMA. Coagulation tubes are filled with buffered tri-sodium citrate solution.
- Phlebotomy Order of Draw-Tube Color and Dept.
- Yellow. Microbiology - SPS - Blood Cultures - Invert 8-10 times.
- Light Blue. Coagulation - NaC - Platelet Function, Routine Coagulation - Invert 3-4 times.
- Gold, Tiger Top, Red/Yellow/Black.
- Green, Light Green, Green/Gray.
Red-Top Tube: This tube is a plain VACUTAINER® containing no anticoagulant and is used for collection of serum for selected chemistry tests as well as clotted blood for immunohematology.
- Plasma. This is the fluid that is removed after centrifugation of anticoagulated whole blood. The exact nature of the plasma. depends on which anticoagulant is used: EDTA plasma.
- Differ from light blue tops because they provide a blood to anticoagulation ratio of 4 to 1. Contains Heparin (anticoagulant) combined with lithium, sodium, or ammonium ion. Primarily used for chemistry tests performed on whole blood or plasma. Why are light green tubes used for potassium determinations?
- Erythrocytes, leukocytes (white blood cells) and thrombocytes (platelets) are stable in EDTA anticoagulated blood for up to 24 hours. Preparation of blood smears should be done within 3 hours after blood collection. EDTA tubes are used for testing whole blood in the clinical laboratory.
- TUBE COLOR. TESTS.
- LAVENDER. CBC, ESR, HgbA1C, Blood Bank (must have within 24 hrs),
- LIGHT BLUE. PT/INR, COAG FACTOR, FIBRiNOGEN, D-DIMER, APTT(must reach lab within 4 hrs or freeze)
- RED/GOLD CENTER (SST)
- RED/BLACK CENTER.
- LIGHT GREEN.
- ROYAL BLUE.
- GRAY. Sterile blood collection tubes for laboratory procedures requiring plasma or whole blood chemistry procedures where glycolytic inhibition of specimen is required. The anti-glycolytic properties prevent the blood cells from using the glucose in the sample.
- Last draw - additive tubes in this order:
- SST (red-gray or gold top). Contains a gel separator and clot activator.
- Sodium heparin (dark green top)
- PST (light green top). Contains lithium heparin anticoagulant and a gel separator.
- EDTA (lavender top)
- ACDA or ACDB (pale yellow top).
- Oxalate/fluoride (light gray top)
- Lavender-Top Tube - EDTA: EDTA is the anticoagulant used for most hematology procedures. Its primary use is for the CBC and individual components of the CBC. The larger (6ml) tube is used for blood bank procedures.
Updated: 25th November 2019