What is a colorimetric analysis?
Colorimetric analysis is a method of determining the concentration of a chemical element or chemical compound in a solution with the aid of a color reagent. It is applicable to both organic compounds and inorganic compounds and may be used with or without an enzymatic stage.
Colorimeter (chemistry) A colorimeter is a device used in colorimetry. In scientific fields the word generally refers to the device that measures the absorbance of particular wavelengths of light by a specific solution.
- Theory: Transmittance and Absorbance. Transmittance (T) is defined as the fraction of incident light which is transmitted, ie, passes through, a sample. Thus, T = I/Io, where Io equals the intensity of light which strikes the sample and I is the intensity of light after passing through the sample.
- A spectrophotometer is an instrument that measures the amount of photons (the intensity of light) absorbed after it passes through sample solution. With the spectrophotometer, the amount of a known chemical substance (concentrations) can also be determined by measuring the intensity of light detected.
- Absorbance is a unitless measure of the amount of light of a particular wavelength that passes through a volume of liquid, relative to the maximum possible amount of light available at that wavelength.
Updated: 2nd October 2019