6th December 2019

utoledo
16

What does a buffer do in PCR?

PCR buffer is necessary to create optimal conditions for activity of Taq DNA polymerase. Buffers often contain Tris-Hcl, KCl, and sometimes MgCl2. PCR Page 4 Polymerase Chain Reaction, 12/2004 4 buffers are often available in 10X concentration and are sometimes Taq formulation-specific.

Also question is, why DNTP is used in PCR?

The purpose of the deoxynucleotide triphosphates (dNTPs) is to supply the “bricks.” Since the idea behind PCR is to synthesize a virtually unlimited amount of a specific stretch of double-stranded DNA, the individual DNA bases must be supplied to the polymerase enzyme.

What is a cofactor in PCR?

An essential cofactor for the DNA polymerase in PCR is Magnesium. chloride.Mg2+ is a cofactor for all DNA polymerases, including reverse transcriptase. It binds to the phosphate groups of the dNTPs. The main 1:1 binding agent for magnesium ion is the high concentration of dNTPs in the reaction.

What is the role of Taq polymerase in PCR?

T. aquaticus is a bacterium that lives in hot springs and hydrothermal vents, and Taq polymerase was identified as an enzyme able to withstand the protein-denaturing conditions (high temperature) required during PCR. Therefore, it replaced the DNA polymerase from E. coli originally used in PCR.
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