2nd October 2019

gbiosciences
13

How does TE buffer protect DNA?

The TE Buffer is a common molecular biology buffer used for protection of DNA and RNA from degradation. The Tris buffering agent and EDTA metal chelating properties help protect DNA and RNA.

Why DNA extraction buffer is used?

Lysis buffer. A lysis buffer is a buffer solution used for the purpose of breaking open cells for use in molecular biology experiments that analyze the compounds of the cells (e.g. western blot). Most lysis buffers contain salts (e.g. Tris-HCl or EDTA) to regulate the acidity and osmolarity of the lysate.

Why Tris buffer is used?

Tris buffers are widely used for DNA agarose electrophoresis. The two main buffers are TBE (Tris borate/EDTA) and TAE (Tris acetate/EDTA). Although there are some differences in the resolution of different forms of DNA and their mobility during electrophoresis, these Tris buffers can generally be used interchangeably.
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