Annealing of primers. To copy DNA, polymerases require a short sequence called a primer. The PCR uses two primers, each complementary to opposite strands of the region of DNA, which have been denatured by heating.
Accordingly, which strand does the forward primer bind to?
The forward primer attaches to the start codon of the template DNA (the sense strand), while the reverse primer attaches to the stop codon of the complementary strand of DNA (the anti-sense strand). The 5' ends of both primers bind to the 3' end of each DNA strand.
What is the sense and antisense strand?
It is the DNA antisense strand which serves as the source for the protein code, because, with bases complementary to the DNA sense strand, it is used as a template for the mRNA. Since transcription results in an RNA product complementary to the DNA template strand, the mRNA is complementary to the DNA antisense strand.
What is the primer design?
A primer is a short synthetic oligonucleotide which is used in many molecular techniques from PCR to DNA sequencing. These primers are designed to have a sequence which is the reverse complement of a region of template or target DNA to which we wish the primer to anneal. Analysis of primer sequences.