0.1M PBS (1X) solution: To make 1 L, add 100 ml 10X PBS solution to 900 ml distilled water and mix. Check pH is 7.4. Store at room temperature. 30% sucrose solution: To make 100 ml, dissolve 30 g sucrose in about 70 ml 0.1M PBS solution; once dissolved, make up volume to 100 ml total by adding 0.1M PBS solution.
What is in a sucrose solution?
Sucrose is the chemical name for table sugar. It consists of a combination of glucose and fructose and is usually obtained from sugar cane or sugar beets. A solution is a liquid, usually water, with a solid dissolved in it.
How do you make a sugar solution?
Here is the recipe for making hummingbird nectar:
- Mix 4 parts water to 1 part table sugar in a pan. For example, use 1 cup sugar to 4 cups water.
- Bring to a boil then remove from the heat.
- Cover and allow to cool before using or pouring into the storage bottle.