28th October 2019

nature

16

How do I make a 30% sucrose solution?

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0.1M PBS (1X) solution: To make 1 L, add 100 ml 10X PBS solution to 900 ml distilled water and mix. Check pH is 7.4. Store at room temperature. 30% sucrose solution: To make 100 ml, dissolve 30 g sucrose in about 70 ml 0.1M PBS solution; once dissolved, make up volume to 100 ml total by adding 0.1M PBS solution.

What is in a sucrose solution?

Sucrose is the chemical name for table sugar. It consists of a combination of glucose and fructose and is usually obtained from sugar cane or sugar beets. A solution is a liquid, usually water, with a solid dissolved in it.

How do you make a sugar solution?

Here is the recipe for making hummingbird nectar:

Mix 4 parts water to 1 part table sugar in a pan. For example, use 1 cup sugar to 4 cups water.
Bring to a boil then remove from the heat.
Cover and allow to cool before using or pouring into the storage bottle.

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What is 1% glucose solution?

Standard solutions in general contain a known amount of substance dissolved in a known quantity of another substance. Usually, a standard glucose solution refers to a 1-percent glucose solution. Preparing a 1-percent standard glucose solution involves dissolving 1 g of glucose in 100 ml of water.

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Is sugar solution a homogeneous or heterogeneous mixture?

Sugar dissolves and is spread throughout the glass of water. The sand sinks to the bottom. The sugar-water is a homogenous mixture while the sand-water is a heterogeneous mixture. Both are mixtures, but only the sugar-water can also be called a solution.

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What does 5% dextrose mean?

D5W (5% dextrose in water), which consists of 278 mmol/L dextrose. D5NS (5% dextrose in normal saline), which, in addition, contains normal saline (0.90% w/v of NaCl). D5 1/2NS 5% dextrose in half amount of normal saline (0.45% w/v of NaCl).

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What is meant by the concentration of the solution?

In chemistry, concentration refers to the amount of a substance per defined space. Another definition is that concentration is the ratio of solute in a solution to either solvent or total solution. However, the solute concentration may also be expressed in moles or units of volume.

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What is phosphate buffer used for?

Phosphate buffered saline (abbreviated as PBS) is a buffer solution commonly used in biological research. It is a salty solution containing sodium chloride, sodium phosphate, and (in some formulations) potassium chloride and potassium phosphate. The buffer helps to maintain a constant pH.

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Why is PBS used?

Phosphate buffered saline (PBS) is a buffer solution commonly used in biological research. The buffer helps to maintain a constant pH. Generally a pH of 7.4 is maintained. The osmolarity and ion concentrations of the solution usually match those of the human body.

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What is Dpbs?

DPBS is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. DPBS is used to to irrigate, wash, and dilute mammalian cells. Phosphate buffering maintains the pH in the physiological range.

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What is in Dpbs?

Dulbecco's phosphate-buffered saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents.

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What is in PBS?

Phosphate-buffered saline (abbreviated PBS) is a buffer solution commonly used in biological research. It is a water-based salt solution containing disodium hydrogen phosphate, sodium chloride and, in some formulations, potassium chloride and potassium dihydrogen phosphate.

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What does PBS do to cells?

Phosphate buffered saline (PBS) is a balanced salt solution commonly used in cell culture and immunofluorescene studies. The purpose of PBS is to maintain the 1) constant pH and the 2) osmolarity of the cells. The pH of most biological materials fall between 7 to 7.6.

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What is the concentration of 1x PBS?

Ready to use 1X PBS buffer is a solution with a phosphate buffer concentration of 10mM, 2.7mM potassium chloride, 137mM sodium chloride, and 1.76mM potassium phosphate. pH 7.4 ±0.2 (25°C).

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What is a biological buffer?

Biological Buffers: Reviewing the Basics. Basically, buffers are molecules that donate or accept protons to resist changes in pH as acids or bases are added to the solution. A buffer consists of a weak acid and its conjugate base, or a weak base and its conjugate acid.

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Is blood a buffer?

Other pH-Buffer Systems in the Blood. Other buffers perform a more minor role than the carbonic-acid-bicarbonate buffer in regulating the pH of the blood. During exercise, hemoglobin helps to control the pH of the blood by binding some of the excess protons that are generated in the muscles.

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What are the uses of buffers in biochemistry?

Buffers are aqueous systems that resist changes in pH as acid or base is added. They are usually composed of a weak acid and its conjugate base. Biological buffers, mixture of weak acids (the proton donors) and their conjugate bases (the proton acceptors), help maintain biomolecules in optimal ionic state of pH 7.

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How are buffers made?

A buffer solution is one which resists changes in pH when small quantities of an acid or an alkali are added to it. An acidic buffer solution is simply one which has a pH less than 7. Acidic buffer solutions are commonly made from a weak acid and one of its salts - often a sodium salt.

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What is a buffer solution and why is it used?

Buffer solutions are used as a means of keeping pH at a nearly constant value in a wide variety of chemical applications. In nature, there are many systems that use buffering for pH regulation. For example, the bicarbonate buffering system is used to regulate the pH of blood.

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What is basic buffer solution?

A buffer solution is one which resists changes in pH when small quantities of an acid or an alkali are added to it. Acidic buffer solutions: An acidic buffer solution is simply one which has a pH less than 7. Acidic buffer solutions are commonly made from a weak acid and one of its salts - often a sodium salt.

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How do buffer solutions work?

A buffer is simply a mixture of a weak acid and its conjugate base or a weak base and its conjugate acid. Buffers work by reacting with any added acid or base to control the pH. Here the buffer also serves to neutralize the base.

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What is in a sucrose solution?

Sucrose is the chemical name for table sugar. It consists of a combination of glucose and fructose and is usually obtained from sugar cane or sugar beets. A solution is a liquid, usually water, with a solid dissolved in it.

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