21st October 2019

sciencelearn
14

How DNA fragments are separated by gel electrophoresis?

How are DNA fragments separated using gel electrophoresis? A solution of DNA molecules is placed in a gel. Because each DNA molecule is negatively charged, it can be pulled through the gel by an electric field. Small DNA molecules move more quickly through the gel than larger DNA molecules.

Accordingly, what does the position of the bands in an electrophoresis gel indicate?

The gel matrix acts as a sieve: smaller DNA molecules migrate faster than larger ones, so DNA molecules of different sizes separate into distinct bands during electrophoresis. To visualise the DNA fragments we added the staining agent Ethidium Bromide to the gel and the buffer solution.

Why do smaller fragments move further in gel electrophoresis?

Separating the fragments. The electrical current is then turned on so that the negatively charged DNA moves through the gel towards the positive side of the gel. Shorter lengths of DNA move faster than longer lengths so move further in the time the current is run.

What is responsible for the movement of the DNA in gel electrophoresis?

Gel electrophoresis is a procedure that separates molecules on the basis of their rate of movement through a gel under the influence of an electrical field. DNA is a negatively charged molecule, so it will move toward the positive pole of the gel when a current is applied.
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